When performing a compression smear, what is placed over the aspirate?

In the procedure of a compression smear, a second spreader slide is used over the aspirate. This technique involves placing one slide on top of the aspirate to flatten and spread the sample evenly. It is important because it allows for optimal cell distribution, which is crucial for adequate examination under a microscope. The spreading action helps to create a thin, uniform layer of cells on the slide, facilitating clearer visualization and better cytological evaluation.

Utilizing a second spreader slide rather than other options ensures that you achieve the correct smear thickness, which is essential for accurate diagnosis. The use of a cover slip, sterile tube, or a sample of fluid would not fulfill the need for thin, spread samples that are necessary for cytological assessment.