What is the primary criticism of insufficient stain time in blue/purple stains?

The primary criticism of insufficient stain time in blue/purple stains is that it leads to poor chromatin visibility. When the staining process is rushed or not given enough time, the dye cannot adequately penetrate the cellular structures, particularly the chromatin within the nucleus. This results in the chromatin appearing pale or poorly defined, making it difficult to assess its morphology and any potential abnormalities. Adequate staining time is crucial for ensuring that the chromatin is sufficiently highlighted, which is necessary for accurate evaluation in cytological examinations.

Although some options mention effects like dehydration or over-staining, these aspects are not directly related to the consequences of insufficient staining time when it comes to blue/purple stains. Proper chromatin visibility is essential for effective diagnosis and interpretation of cytological samples.