During the making of a combination smear, where should the second slide be placed after it meets the aspirate?

In the procedure of creating a combination smear, positioning the second slide correctly is crucial to ensure that the cell distribution is optimal for examination. Placing the second slide at a right angle to the prep slide allows for effective transfer and spreading of the aspirated material. This right-angle approach facilitates an even distribution of the sample across the surface of the prep slide, which is important for obtaining a well-prepared smear that accurately represents the cellular details needed for analysis.

By utilizing this technique, the risk of clumping or uneven spreading is minimized, which is essential for obtaining clear and interpretable results. The right angle helps prevent excess pressure on the cells, maintaining their integrity and overall morphology, aiding in enhanced evaluation during cytological examination.